资讯中心 >产品文献集>Microarray Technology (15)    相关产品：

  ✔本篇论文使用华联产品：OEM Array  
 Applied Mechanics and Materials . 2013 Jan. doi: 10.4028/www.scientific.net/AMM.284-287.315.
 Investigation of Hybridization Efficiency of a Sequence-Orientated Coaxial DNA Probe Microarryed on Biochips Using Atomic Force Microscope 
 Dan-Kai Yang, Yane-Shu Lin, Jun-Yi Chen, Jui-Chuang Wu
  Abstract
Two sequence-inversed probes were microarrayed on glass slides to study the hybridization efficiency with their DNA targets. The sequences of two targets were designed to be fully complementary to their shared DNA probe in a coaxial stacking configuration. The enhancement of the hybridization efficiency was investigated through the comparison between the stacking and individual hybridization configurations. AFM was used to measure the depths of two probes at different steps of hybridization. The results indicated that the depths increased as the hybridization proceeded. Probe#1, pre-hybridizing close to the chip surface, obtained a thicker depth than the other probe pre-hybridizing away from the chip surface, Probe#2. A hypothesis was proposed to explain how the depth variation was associated with the observed hybridization efficiency.
   

  ✔本篇论文使用华联产品：  
 Journal of Laboratory Automation. 2012, 17(6):405-407. doi: 10.1177/2211068212463689.
 Lab Automation Services
 
 
 Stephen Hughes
  Abstract

   

  ✔本篇论文使用华联产品：  
 Neurology Today. 2011, 11(19): 24-27. doi: 10.1097/01.NT.0000407216.70062.aa.
 Evidence Report: Genetic, Metabolic Screening Useful in children with Intellectual Development Deficits
 
 
 KURT SAMSON
  Abstract
Although there are challenges yet to be overcome in the diagnostic evaluation of children with unexplained global developmental delay or intellectual disability (GDD/ID), genetic and metabolic screening tests are increasingly useful, according to a review by the AAN Quality Standards Subcommittee and the Practice Committee of the Child Neurology Society. The review was published in the Sept. 28 online edition of Neurology. In an interview with Neurology Today, AAN subcommittee panelist David J. Michelson, MD, assistant professor of neurology and pediatrics at the Loma Linda University School of Medicine in California, discussed the fi ndings and their implications for clinicians.
   

  ✔本篇论文使用华联产品：  
 Nature Methods. 2010, 7:181-186. doi:10.1038/nmeth0310-181.
 Epigenome: mapping in motion
 
 
 Monya Baker
  Abstract
As high-throughput techniques accelerate mapping of epigenetic marks, researchers are racing to find the biological meaning of these marks.
   

  ✔本篇论文使用华联产品：  
 Nature methods. 2010, 7(9):687-92. doi: 10.1038/nmeth0910-687.
 MicroRNA profiling: separating signal from noise
 
 
 Monya Baker
  Abstract
MicroRNAs may be small, but these noncoding RNAs that regulate gene expression are creating a big stir. Finding differences in the expression of microRNAs between, say, healthy and diseased cells could potentially be used to diagnose diseases or to assess treatment effects. If researchers can understand how they work, microRNAs could provide tools for manipulating genes, not to mention help to untangle how genes are regulated.At first glance, studying microRNAs seems more manageable than studying the menagerie of other types of RNA. Typical expression profiling experiments for protein-coding genes examine thousands of molecules; those for microRNAs examine hundreds. But researchers are still figuring out the most reliable ways to measure these important molecules. The most common techniques for profiling microRNAs are deep sequencing, microarrays and quantitative real-time PCR (qPCR). All are supported by several commercial offerings. Though specific products and techniques vary, researchers generally agree on the relative strengths and weaknesses of the platforms. The best choice depends on the application, says Muneesh Tewari, who studies microRNAs at the Fred Hutchinson Cancer Research Center. “It's a balance of cost, precision, accuracy and sample quantity,” he says. “If the purpose is to screen a bunch of samples to find a few microRNAs that change and you can tolerate a false negative, then the microarray may be the best platform. If the purpose is to detect microRNAs where the sample amount is limiting, then qPCR has better sensitivity, and if you are trying to see different isoforms or very similar microRNAs, then sequencing is going to be the best approach.
   

  ✔本篇论文使用华联产品：Human OneArray, Mouse OneArray  
 Methods Mol Biol. 2009;590:165-76.
 Methylated DNA Immunoprecipitation and Microarray-Based Analysis: Detection of DNA Methylation in Breast Cancer Cell Lines
 
 
 Tim H. M. Huang, and Pearlly S. Yan, Yu-I Weng
  Abstract
The methylated DNA immunoprecipitation microarray (MeDIP-chip) is a genome-wide, high-resolution approach to detect DNA methylation in whole genome or CpG (cytosine base followed by a guanine base) islands. The method utilizes anti-methylcytosine antibody to immunoprecipitate DNA that contains highly methylated CpG sites. Enriched methylated DNA can be interrogated using DNA microarrays or by massive parallel sequencing techniques. This combined approach allows researchers to rapidly identify methylated regions in a genome-wide manner, and compare DNA methylation patterns between two samples with diversely different DNA methylation status. MeDIP-chip has been applied successfully for analyses of methylated DNA in the different targets including animal and plant tissues. Here we present a MeDIP-chip protocol that is routinely used in our laboratory, illustrated with specific examples from MeDIP-chip analysis of breast cancer cell lines. Potential technical pitfalls and solutions are also provided to serve as workflow guidelines.
   

  ✔本篇论文使用华联产品：Experimental Accessories  
 Journal of the Taiwan Institute of Chemical Engineers. 2011, 42(1):5-12.
 Effect of co-axially hybridized gene targets on hybridization efficiency of microarrayed DNA probes
 
 
 Kai Ren Jiang, Jie-Len Huang, Chia-Chun Chen, Hung-Ju Su, Jui-Chuang Wu
  Abstract
The effect of relative size of two co-axially hybridized gene targets on the hybridization efficiency was studied for two DNA probe configurations and various probe concentrations. Each of two sets of microarrayed probes contained a pair of DNA probes and a pair of their complementary samples labeled with two distinct fluorescent dyes. The sequence of each probe is especially designed so that two targets are simultaneously complementary to two adjacent sections of the probe. The molecular steric effect on the hybridization efficiency is investigated by comparing the dye signals between configurations of one-target and two-target hybridization scenarios. The results show that a low probe concentration gives better hybridization efficiency and the first-hybridization conducted by a shorter-size DNA target improves the hybridization efficiency of the second target coupling onto the same probe.
   

  ✔本篇论文使用华联产品：Experimental Accessories  
 Acta Biomaterialia. 2010, 6(4):1462-70. doi: 10.1016/j.actbio.2009.10.001.
 A novel three-dimensional aerogel biochip for molecular recognition of nucleotide acids.
 
 
 Yen Kuang Li, Den-Kai Yang, Yun-Chu Chen, Hung-Ju Su, Jui-Chuang Wu, Yui Whei Chen-Yang.
  Abstract
Mesoporous aerogel was produced under regular atmospheric conditions using the sol-gel polymerization of tetraethyl orthosilicate with an ionic liquid as both solvent and active agent. This was then used to build a three-dimensional structure to recognize nucleotide acids. Fourier transformation infrared spectroscopy, scanning electron microscopy, (29)Si solid-state nuclear magnetic resonance, and Brunauer-Emmett-Teller instruments were used to characterize this 3D aerogel, demonstrating that it had high porosity and large internal networking surface area that could capture nucleotide acids. The functionality of molecular recognition on nucleotide acids was demonstrated by immobilizing an oligonucleotide to probe its DNA target and confirming the tagged fluorescent signals by confocal laser scanning microscopy. The results indicated that the as-prepared 3D bioaerogel was capable of providing a very large surface area to capture and recognize human gene ATP5O.
   

  ✔本篇论文使用华联产品：  
 Biotechnology Annual Review. 2008, 14:29-61. doi: 10.1016/S1387-2656(08)00002-1.
 Gene expression microarray data analysis demystified
 
 
 Peter C. Roberts
  Abstract
The increasing use of gene expression microarrays, and depositing of the resulting data into public repositories, means that more investigators are interested in using the technology either directly or through meta analysis of the publicly available data. The tools available for data analysis have generally been developed for use by experts in the field, making them difficult to use by the general research community. For those interested in entering the field, especially those without a background in statistics, it is difficult to understand why experimental results can be so variable. The purpose of this review is to go through the workflow of a typical microarray experiment, to show that decisions made at each step, from choice of platform through statistical analysis methods to biological interpretation, are all sources of this variability.
   

  ✔本篇论文使用华联产品：Experimental Accessories  
 Journal of the Chinese Institute of Chemical Engineers. 2008, 39(3):187-193. doi: 10.1016/j.jcice.2007.12.012.
 Enhancement of target-DNA hybridization efficiency by pre-hybridization on sequence-orientated micro-arrayed probes.
 
 
 Dan-Kai Yang, Jie-Len Huang, Chia-Chun Chen, Hung-Ju Su, Jui-Chuang Wu
  Abstract
The enhancement of hybridization efficiency of deoxyribonucleic acid (DNA) targets using oligonucleotide pre-hybridization is studied on two sequence-inversed micro-arrayed probes. The sequences for pre-hybridizing both oligo and target DNA are designed to be fully complementary with their shared DNA probe in a coaxial stacking configuration; i.e. they hybridize immediately alongside each other along the continuous complement probe strand. The pre-hybridizing oligo and target DNA are differentiated by being labeled with two distinct fluorescent dyes, and the cooperative effect on hybridization efficiency is investigated through the comparison of the stacking and individual hybridization configurations based on the detection signals of the labeling dyes. The results show that the pre-hybridization of a DNA oligo enhances the subsequent hybridization efficiency of the target-DNA coupling onto the same probe. The efficiency is enhanced if the hybridization position occurs at a site close to the substrate surface.
   

  ✔本篇论文使用华联产品：  
 Asia Pacific Biotech News. 2007, 11(12):830-834. doi: 10.1142/S0219030307000857.
 ITRI: Bridging Innovative Biomedical Engineering R&D to Bioeconomy
 
 
 Chung-Cheng Liu
  Abstract
This article shortly explains about bridging innovative biomedical engineering R&D to bioeconomy in Taiwan.
   

  ✔本篇论文使用华联产品：  
 Technovation. 2006, 26(1):104-110.
 Stimulating new industries from emerging technologies: challenges for the public sector
 
 
 Yee-Yeen Ch, Shih-Chang Hung
  Abstract
Stimulating new industries from emerging technologies is central to successful high-tech based economic growth, employment, competition and sustainability in modern market economies. The Taiwanese experiences in developing new technology-based industries (e.g. integrated circuits, personal computers, notebooks, scanners, and TFT LCDs) illustrate some of the ways policy makers can shape the development of emerging technologies into new industries. These ways of actions are manifold, but at least policy attentions to three key mechanisms are critical. These mechanisms encourage partnership in the commercialization process, foster entrepreneurship and venture initiatives in the innovation system, and sustain commercialization and the creation of new firms. The study of Industrial Technology Research Institute in Taiwan on biochips and nanotechnology further shows how the policy makers can build a statutory body to effectively address the functions of three mechanisms as a whole.
   

  ✔本篇论文使用华联产品：  
 Expert Rev Mol Diagn. 2006, 6(4):535-50. doi:10.1586/14737159.6.4.535.
 Microarray RNA transcriptional profiling: Part I. Platforms, experimental design and standardization
 
 
 Farid E Ahmed
  Abstract
This review summarizes, in a balanced and comprehensive manner, the various components of microarrays and their types, substrate architecture, platforms for microarray probe implementation, standardizations and confounders. The review is intended to familiarize the beginner with the principles of experimental design and the selection of an appropriate microarray platform. This parallel technology has revolutionized transcriptomic approaches to data profiling and has a major role in the identification of expressed genes, classification and diagnosis studies. The technology is still evolving and guidelines for standardization and reporting have been developed and are being improved.
   

  ✔本篇论文使用华联产品：  
 Thermochimica Acta. 2005, 433: 83–87. doi: 10.1016/j.tca.2005.01.072.
 Thermodynamics and mechanism of ssDNA hybridization below the melting temperature by isothermaltitration calorimetry
 
 
 An-Cheng Liu, Liang-Yu Chen, Chung-Fan Chiou, Hung-Ju Richard Su, Yu-Chia Chan, Pei-Hsuan Tasi, Shean-Jen Chen, Wen-Yih Chen
  Abstract
This investigation presents measurements of isothermal ssDNA oligomer hybridization enthalpy at various DNA length, GC contents, temperatures, and salt concentrations by isothermal titration calorimetry (ITC) at temperatures below the melting temperature of the DNA. The study aimed to reveal the hybridization mechanism of ssDNA for gene chip applications. The role of hydrogen bonds between complementary bases, the stacking forces between bases, the electrostatic repulsion between strands, the dehydration and conformational rearrangement of ssDNA in hybridization are estimated from the interaction enthalpy.
   

  ✔本篇论文使用华联产品：  
 Chemical Physics Letters. 2004, 397: 429–434. doi:10.1117/12.647449.
 An investigation into the influence of secondary structures on DNA hybridization using surface plasmon resonance biosensing
 
 
 Fan-Ching Chien, Jih-Shiou Liu, Huang-Ju Su, Li-An Kao, Chung-Fan Chiou, Wen-Yih Chen, Shean-Jen Chen
  Abstract
This study utilizes a surface plasmon resonance (SPR) biosensing and a theoretical secondary structure calculation to investigate the influence of secondary structures on the DNA hybridization. It is found that the SPR angular shifts associated with the three pairs of 60mer oligonucleotides with prominent secondary structures are lower than those observed for the two pairs of oligonucleotides with no obvious secondary structures. It is also determined that increasing the DNA hybridization temperature from 35 to 45 °C reduces secondary structure effects. On the hybridization with mixture target oligonucleotides, the SPR results demonstrate that secondary structures interfere significantly.