产品资讯 > 模式植物 miRNA 芯片 Model Plant miRNA OneArray® v3

 
  human microarray for gene expression   模式植物 miRNA 芯片 v3 是参考 Sanger miRBase 资料库 release 17 的序列进行探针设计, 并以自有的高速布放技术进行芯片生产。v3 版中涵盖阿拉伯芥、水稻、大豆等七种常见模式植物的 miRNA 探针序列, 于 20119 月上市。
  
 
 
 

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  基 因 探 针

模式植物 miRNA 芯片 v3 是参考 Sanger miRBase 资料库 release 17 的序列进行探针设计, 共计 1,018 个序列,可对应到 1,817 个成熟的 miRNA。 在探针设计的过程,依据各序列的特性,进行严格 Tm 值的把关及调整。 在每一芯片上,每一个 miRNA 序列具有 3 个重覆的探针。



  控 制 探 针

为确保芯片数据品质,华联技术团队经过一连串的测试及验证, 设计一系列的品质控制探针,以监控完整的芯片实验步骤,包含矩阵校准标记探针、 阳性控制探针及阴性控制探针。
 

使用MPmiOA文献 ( 1 )

 Journal of Experimental Botany. 2013, 64(1):303-15. doi: 10.1093/jxb/ers333.
 Identification and profiling of arsenic stress-induced microRNAs in Brassica juncea 
 Ashish Kumar Srivastava, S.F. D’Souza, Sudhakar Srivastava, Penna Suprasanna
  Abstract
MicroRNAs (miRNAs) constitute a novel mechanism of gene regulation affecting plant development, growth, and stress response. To study the role of miRNAs in arsenic (As) stress, microarray profiling of miRNAs was performed in Brassica juncea using a custom Phalanx Plant OneArray containing 381 unique miRNA probes representing 618 miRNAs from 22 plant species. miRNA microarray hybridization of roots exposed to As for 1 h and 4 h revealed that a total of 69 miRNAs belonging to 18 plant miRNA families had significantly altered expression. The As-responsive miRNAs also exhibited a time- and organ-dependent change in their expression. Putative target prediction for the miRNAs suggested that they regulate various developmental processes (e.g. miR156, miR169, and miR172), sulphur uptake, transport, and assimilation (miR395, miR838, and miR854), and hormonal biosynthesis and/or function (e.g. miR319, miR167, miR164, and miR159). Notable changes were observed in the level of auxins [indole-3-acetic acid (IAA), indole-3- butyric acid, and naphthalene acetic acid], jasmonates [jasmonic acid (JA) and methyl jasmonate], and abscisic acid. The exogenous supply of JA and IAA improved growth of plants under As stress and altered expression of miR167, miR319, and miR854, suggesting interplay of hormones and miRNAs in the regulation of As response. In conclusion, the present work demonstrates the role of miRNAs and associated mechanisms in the plant’s response towards As stress.