产品资讯 > 酵母菌表达谱芯片 Yeast OneArray® v1 > 技术支援 \ 阳性控制探针

 
  Yeast microarray for gene expression   酵母菌表达谱芯片 v1 的探针是采用 Operon Yeast Genome Array-Ready Oligo Set (AROS) v1.1Yeast Brown Lab Oligo Extension (YBOX) v1.0 探针组资讯所设计, 为长链 70 个碱基寡核酸探针。 酵母菌表达谱芯片 v120103 月上市。 探针皆设计在离 3’750 碱基以内的序列,适合用来进行基因表现的研究。

 
 

  阳性控制探针
 
为了监测样品备制备过程中 RNA 放大的效率与杂交效率等, 在酵母菌表达谱芯片中,包含 Operon Yeast Genome Array-Ready Oligo Set (AROS) v1.1 探针组中所设计的 12 种阳性探针, 可作为实验的控制探针。
 
   
探针编号 基因名称 基因功能说明
YLL039C_01 Operon positive control_UBI4 Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response.
YBL092W_01 Operon positive control_RPL32 Protein component of the large (60S) ribosomal subunit, has similarity to rat L32 ribosomal protein; overexpression disrupts telomeric silencing.
YBR181C_01 Operon positive control_SSA4 Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation.
YFL039C_01 Operon positive control_ACT1 Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions.
YIL115C_01 Operon positive control_NUP159 Subunit of the nuclear pore complex that is found exclusively on the cytoplasmic side, forms a subcomplex with Nup82p and Nsp1p, required for mRNA export.
YJL052W_01 Operon positive control_TDH1 Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall.
YJL061W_01 Operon positive control_NUP82 Subunit of the nuclear pore complex (NPC), forms a subcomplex with Nup159p and Nsp1p, interacts with Nup116p and is required for proper localization of Nup116p in the NPC.
YJR009C_01 Operon positive control_TDH2 Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall.
YLL026W_01 Operon positive control_HSP104 Heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation.
YLR212C_01 Operon positive control_TUB4 Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body.
YPL016W_01 Operon positive control_SWI1 Subunit of the SWI/SNF chromatin remodeling complex, which regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2.