产品资讯 > 酵母菌表达谱芯片 Yeast OneArrayR v1

 
  Yeast microarray for gene expression   酵母菌表达谱芯片 v1 的探针是采用 Operon Yeast Genome Array-Ready Oligo Set (AROS) v1.1 与 Yeast Brown Lab Oligo Extension (YBOX) v1.0 探针组资讯所设计， 为长链 70 个碱基寡核酸探针。 酵母菌表达谱芯片 v1 于 2010 年 3 月上市。 探针皆设计在离 3’ 端 750 碱基以内的序列，适合用来进行基因表现的研究。

 
 
 
酵母菌表达谱芯片 v1 探针内容
探针种类 探针数
  基因探针设计资料库： 6,958
          - Operon Yeast Genome Array-Ready Oligo Set (AROS) v1.1
          - Yeast Brown Lab Oligo Extension (YBOX) v1.0
  控制探针数 684
  总探针数 7,642


  基 因 探 针

酵母菌表达谱芯片 v1 是由华联的技术团队与中央研究院所共同合作生产， 探针的设计及收集是参考国际上较普遍使用的 Operon Yeast Genome Array-Ready Oligo Set (AROS) v1.1 与 Yeast Brown Lab Oligo Extension (YBOX) v1.0 的探针组资讯， 布放 70 个碱基寡核酸的长链探针。 所有的探针皆分布在离基因 3’ 端 750 碱基以内的序列， 适合用来进行基因表现的研究。 大鼠表达谱芯片是参考国际公认之 RefSeq 及 Ensembl 序列资料库， 设计长链 60 个碱基寡核酸 (sense-strand) 的基因探针， 内容主要以蛋白质编码基因 (protein-coding gene) 为主， 提供高基因覆盖率并且每一个探针对其来源资料库的目标基因皆具高度专一性，避免非目标基因的杂交影响。



  控 制 探 针

为确保芯片数据品质，华联技术团队经过一连串的测试及验证，设计一系列的品质控制探针， 以监控完整的芯片实验步骤，包含样本 RNA 完整性、样本 RNA 放大流程、萤光标记效率、芯片杂交、芯片扫描等。
 

使用YOA文献 ( 4 )

 Journal of Integrative Plant Biology. 2014 Jan 14. doi: 10.1111/jipb.12169.
 Identification of a novel pathway involving a GATA transcription factor in yeast and possibly plant Zn uptake and homeostasis 
 Matthew J. Milner, Nicole S. Pence, Jiping Liu, Leon V.Kochian
  Abstract
To gain a better understanding of the regulation of Zn homeostasis in plants and the degree of conservation of Zn homeostasis between plants and yeast, a cDNA library from the Zn/Cd hyperaccumulating plant species, Noccaea caerulescens, was screened for its ability to restore growth under Zn limiting conditions in the yeast mutant zap1▵. ZAP1 is a transcription factor that activates the Zn dependent transcription of genes involved in Zn uptake, including ZRT1, the yeast high affinity Zn transporter. From this screen two members of the E2F family of transcription factors were found to activate ZRT1 expression in a Zn independent manner. The activation of ZRT1 by the plant E2F proteins involves E2F-mediated activation of a yeast GATA transcription factor which in turn activates ZRT1 expression. A ZRT1 promoter region necessary for activation by E2F and GATA proteins is upstream of two zinc responsive elements previously shown to bind ZAP1 in ZRT1. This activation may not involve direct binding of E2F to the ZRT1 promoter. The expression of E2F genes in yeast does not replace function of ZAP1; instead it appears to activate a novel GATA regulatory pathway involved in Zn uptake and homeostasis that is not Zn responsive.
   

 Journal of Agricultural and Food Chemistry. 2013 Jun 10. doi: 10.1021/jf401831e.
 Tangeretin sensitizes SGS1 deficient cells by inducing DNA damage
 
 
 Shin Yen Chong, Meng-Ying Wu, Yi-Chen Lo
  Abstract
Tangeretin, a polymethoxyflavone found in citrus peel, has been shown to have anti-atherogenic, anti-inflammatory, and anti-carcinogenic properties. However, the underlying target pathways are not fully characterized. We investigated the tangeretin sensitivity of yeast (Saccharomyces cerevisiae) mutants for DNA damage response or repair pathways. We found that tangeretin treatment significantly reduced (p < 0.05) survival rate, induced preferential G1 phase accumulation, and elevated the DNA double-strand break (DSB) signal γH2A in DNA repair-defective sgs1Δ cells, but had no obvious effects on wild-type cells or mutants of the DNA damage checkpoint (including tel1∆, sml1∆ mec1∆, sml1∆ mec1∆ tel1∆, and rad9∆ mutants). Additionally, microarray data indicated that tangeretin treatment up-regulates genes involved in nutritional processing and down-regulates genes related to RNA processing in sgs1∆ mutants. These results suggest tangeretin may sensitize SGS1 deficient cells by increasing a marker of DNA damage, and by inducing G1 arrest and possibly metabolic stress. Thus, tangeretin may be suitable for chemosensitization of cancer cells lacking DSB-repair ability.
   

 Metabolic Engineering. 2012, 14(6):611-22. doi: 10.1016/j.ymben.2012.07.011.
 Xylose isomerase overexpression along with engineering of the pentose phosphate pathway and evolutionary engineering enable rapid xylose utilization and ethanol production by Saccharomyces cerevisiae
 
 
 Hang Zhou, Jing-sheng Cheng, Benjamin Wang, Gerald R. Fink, Gregory Stephanopoulos
  Abstract
Xylose is the main pentose and second most abundant sugar in lignocellulosic feedstocks. To improve xylose utilization, necessary for the cost-effective bioconversion of lignocellulose, several metabolic engineering approaches have been employed in the yeast Saccharomyces cerevisiae. In this study, we describe the rational metabolic engineering of a S. cerevisiae strain, including overexpression of the Piromyces xylose isomerase gene (XYLA), Pichia stipitis xylulose kinase (XYL3) and genes of the non-oxidative pentose phosphate pathway (PPP). This engineered strain (H131-A3) was used to initialize a three-stage process of evolutionary engineering, through first aerobic and anaerobic sequential batch cultivation followed by growth in a xylose-limited chemostat. The evolved strain H131-A3-ALCS displayed significantly increased anaerobic growth rate (0.203±0.006 h?1) and xylose consumption rate (1.866 g g?1 h?1) along with high ethanol conversion yield (0.41 g/g). These figures exceed by a significant margin any other performance metrics on xylose utilization and ethanol production by S. cerevisiae reported to-date. Further inverse metabolic engineering based on functional complementation suggested that efficient xylose assimilation is attributed, in part, to the elevated expression level of xylose isomerase, which was accomplished through the multiple-copy integration of XYLA in the chromosome of the evolved strain.
   

 PLoS One.. 2011, 6(7):e22209. doi: 10.1371/journal.pone.0022209.
 The C-Terminus of Histone H2B Is Involved in Chromatin Compaction Specifically at Telomeres, Independently of Its Monoubiquitylation at Lysine 123
 
 
 Wang CY, Hua CY, Hsu HE, Hsu CL, Tseng HY, Wright DE, Hsu PH, Jen CH, Lin CY, Wu MY, Tsai MD, Kao CF.
  Abstract
Telomeric heterochromatin assembly in budding yeast propagates through the association of Silent Information Regulator (SIR) proteins with nucleosomes, and the nucleosome array has been assumed to fold into a compacted structure. It is believed that the level of compaction and gene repression within heterochromatic regions can be modulated by histone modifications, such as acetylation of H3 lysine 56 and H4 lysine 16, and monoubiquitylation of H2B lysine 123. However, it remains unclear as to whether or not gene silencing is a direct consequence of the compaction of chromatin. Here, by investigating the role of the carboxy-terminus of histone H2B in heterochromatin formation, we identify that the disorderly compaction of chromatin induced by a mutation at H2B T122 specifically hinders telomeric heterochromatin formation. H2B T122 is positioned within the highly conserved AVTKY motif of the αC helix of H2B. Heterochromatin containing the T122E substitution in H2B remains inaccessible to ectopic dam methylase with dramatically increased mobility in sucrose gradients, indicating a compacted chromatin structure. Genetic studies indicate that this unique phenotype is independent of H2B K123 ubiquitylation and Sir4. In addition, using ChIP analysis, we demonstrate that telomere structure in the mutant is further disrupted by a defect in Sir2/Sir3 binding and the resulting invasion of euchromatic histone marks. Thus, we have revealed that the compaction of chromatin per se is not sufficient for heterochromatin formation. Instead, these results suggest that an appropriately arrayed chromatin mediated by H2B C-terminus is required for SIR binding and the subsequent formation of telomeric chromatin in yeast, thereby identifying an intrinsic property of the nucleosome that is required for the establishment of telomeric heterochromatin. This requirement is also likely to exist in higher eukaryotes, as the AVTKY motif of H2B is evolutionarily conserved.